Continuing with the roll-out of its "cornerstone" strategy, American Airlines plans to cut six daily flights from Boston to Washington National airport on 1 November.
American's wholly-owned regional subsidiary American Eagle operates 37-seat Embraer ERJ-135 regional jets on the pairing.
American earlier this year introduced its new strategy of focusing the bulk of its flights in five key markets - Chicago, Dallas, Los Angeles, Miami, and New York.
Explaining American's decision to eliminate the flights a carrier spokesman says: "We are maximising the revenue potential from our limited resources, as we often do, by deploying these planes on new routes which we believe will be more profitable and more appealing as part of our cornerstone strategy in New York."
American is exiting the Boston-Washington market just as JetBlue launches seven new daily shuttle flights on the route 1 November. As part of negotiations of an interline deal unveiled on 31 March, American agreed to transfer eight slot pairs at Washington National to JetBlue.
Both US Airways and Delta offer multiple daily flights between Washington National and Boston.
The interline agreement between American and JetBlue covers 18 JetBlue flights from Boston and New York's John F Kennedy International airport, and 12 of American's international destinations from those two airports.