American Airlines will end its US Airways subsidiary's flight between Charlotte and Rio de Janeiro Galeao in early 2015, as part of the integration of the two carriers' networks.
The decision is "part of the constant evaluation of our overall combined network to ensure we are maximising our fleet and profitability, and matching customer demand", the Fort Worth, Texas-based Oneworld alliance carrier says.
American will continue to serve Rio de Janeiro from Dallas/Fort Worth, Miami and New York JFK.
US Airways operates the Charlotte-Rio flight with either an Airbus A330-200 or Boeing 767-200ER aircraft.
American plans to remove 12 767-200ER aircraft from its fleet in 2014.
US Airways' flight between Charlotte and São Paulo Guarulhos will remain unchanged, says American.
The airline plans to add new seasonal nonstop flights from Charlotte to Barcelona, Lisbon and Manchester (UK) form May, and to Brussels from June.
No other carriers fly between Charlotte and Rio, Innovata schedules show.