Boliviana de Aviacion (BoA) has applied to the US Department of Transportation (DOT) to begin service to Miami and Washington DC via Panama City from May 2013.
The airline will operate the flights from Santa Cruz with Boeing 737-700 aircraft, it says in the filing.
It will first begin operating daily service between Miami and Santa Cruz via Panama City, before starting service to Washington DC "shortly thereafter".
BoA began international service in May 2010 and operates to Buenos Aires and Sao Paulo. It plans to expand international service to Chile, Cuba, Peru and Venezuela besides the USA, says the airline.
BoA says its planned Washington DC service will replace flights previously operated by Bolivian carriers Aerosur and Lloyd Aereo Boliviano (LAB). Aerosur suspended operations in March 2012 after running into financial difficulties while LAB was grounded as a scheduled carrier in 2007.
American Airlines operates non-stop between Santa Cruz and Miami, schedules in Innovata show. No airline operates between Santa Cruz and Washington DC.