Delta Air Lines regional subsidiary Comair's last flight landed at 19:25 on 28 September, after more than three decades of flying.
Comair's last flight was 3025 from Houston Intercontinental to Minneapolis-St. Paul, says Delta.
The Cincinnati-based airline was the first to introduce the 50-seat regional jet in the US - an aircraft that still dominates regional airline fleets - in the early 1990s and had the largest fleet of the small jets in the world for more than a decade.
Delta bought Comair in 1999 and began downsizing the carrier in September 2010. It announced that it would shut down the airline, citing customer expectations and unit costs, this past July.
"I have been, and continue to be a very proud Comair pilot," said Erik Jensen, chairman of the Comair master executive council at the Air Line Pilots Association, in a letter to pilots on 29 September. "Our storied history is one that will live on in the memories of not only our own pilots but also the rest of the industry."