Delta Air Lines will end flights between Cincinnati and Washington Dulles from 2 March 2013.
The 12-times weekly service is flown by ExpressJet as Delta Connection, according to the Atlanta-based carrier.
Delta and its regional partners will operate 110 peak day departures from Cincinnati after the flights end, based on its December schedule.
United Airlines will be the only airline flying between Cincinnati and Dulles once the Delta flights end, according to Innovata schedules.
Separately, Delta will add a third daily flight between Atlanta and London Heathrow from 31 March 2013.