Dutch carrier KLM is to pull out of Liverpool Airport in the UK in March, two years after resuming its link to Amsterdam Schiphol following a 70-year absence.
Peel Airports, which operates Liverpool, said KLM will terminate its Liverpool-Amsterdam service on 31 March.
The airport operator said the decision was made "in spite of good passenger numbers and improving route performance", adding that it will make "very effort" to replace the service.
"We are naturally very disappointed that KLM have taken this decision, however we understand the challenging economic conditions that they and many others in the aviation industry currently operate in," said Peel Airports chief executive Craig Richmond.
"This service has also been important for the Liverpool city region, opening up global connectivity for travel to and from the region and it is for this reason that we will pursue all alternatives as we look for a replacement service to a hub airport."
Liverpool will continue to be linked to Amsterdam Schiphol by EasyJet's thrice-daily service.
KLM Cityhopper resumed flights between Liverpool and Amsterdam on 27 March 2009, using Fokker 70s. Prior to that, the Dutch carrier had not served the route since before the Second World War.
KLM was not immediately available for comment.