Taiwan's TranAsia Airways will commence daily non-stop service on the Taipei Taoyuan-Singapore route from 30 June.
The carrier will operate Airbus A320 aircraft on the route, said Changi Airport Group.
Including TransAsia, six carriers operate a total of 60 weekly flights on the route, it added.
"The commencement of TransAsia's Taipei-Singapore service is timely to meet the strong travel demand by both Taiwan and Singapore residents on this sector, and provides travellers with more options when visiting both cities," said Yam Kum Weng, executive VP for air hub development, Changi Airport.
The other carriers operating on the route are China Airlines, Eva Air, Jetstar Asia, Singapore Airlines and Tiger Airways.