Low-cost carrier Tiger Airways plans to resume flights on the Melbourne-Darwin route from 10 June.
The Singapore-based airline's Australian subsidiary will operate the service six times a week, says Melbourne Airport.
Tiger suspended services to Darwin in October 2008, citing increased fuel costs and high charges at Darwin Airport.
The airline, which operates Airbus A320-family aircraft, also launched flights between Melbourne and Brisbane yesterday. It will operate 19 flights weekly on the route.
"Services to Brisbane bring Tiger's destinations when flying out of Melbourne to 13 - and come June, with the addition of Darwin services, 14, showing the strength not only of the Melbourne market but the Victorian economy as a whole," says Melbourne Airport's CEO Chris Woodruff.