Irkut has started the flight-test programme with the MC-21-300 from the Gromov institute at Moscow's Zhukovsky airfield.
The aircraft undertook a 3h flight on 2 November, the airframer states.
It says the tests assessed stability and control at a range of altitudes from 6,000-12,000m (19,700-39,400ft).
"All aircraft systems operated normally," adds Irkut.
It flew the MC-21 from its Irkutsk base to the Gromov centre on 17 October.